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Windows 2008 R2 / DFS / IIS / "The specified network is no longer available"

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Migrated over to 2008 not all that long ago, all was going OK until last weekend - alot of our clients were experiencing errors, from what I could see it was all relating to a file access problem. 
We put the issue down to our Load Balancer - which we changed the LoadBalancer timeout from 3 seconds to 5. This would hopefully allow the API call to be run, and would give me till Monday to find out what happened!

Since this change IIS logs it has ALOT of sc-win32-status=64errors - which translates to "The specified network is no longer available"

IIS content & config is replicated with DFS. There are alot of small files (<1kb) which are only there for a short time - but as this wasnt an issue last week, could this be the issue now?

I have gone through everything - no joy. The only way I can stop those 64 errors occurring is to disable DFS replication - which defeats the purpose of the Loadbalancer really!

To be honest the DFS side of things was all setup by our DC, however we're all stumped which leaves me in a bit of a mess - any help/advice/opinions would be greatly appreciated!




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