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Cannot add users to folder permissions

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We have a file server (Server 2003 R2 member of a 2003R2 domain) on which I can no longer add users to folder security permissions. When I try to enter a username and click check name it says they can't be found and if I click on locations the AD tree below the domain name won't expand - I just get the domain name and the + box disappears as below.


On the server in Admin Tools ADUC would not work either so I uninstalled and now can't re-install...

I am able to add permissions to files and folders on the server remotely, i.e. browse to the admin share for the relevant drive on the server from my desktop PC and find the file / folder and add permissions that way. After doing this the permissions appear on the server, but I can't add them directly?!?

I'd considered removing the server from the domain and re-adding but the server has a number of user and application shares which would be lost, but I'm concerned it might stop working altogether. 

I've tried SFC /Scannow but don't have the install disks (as I wasn't left them but those who I took over from).

I'd be grateful of some diagnostic help / suggestions to fix the issue

Thanks



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